Efek Neuroprotektor dan Neuroterapi Ekstrak Akar Acalypha indica Linn (Akar Kucing) secara Eks Vivo dan In Vivo


Staff : Ernie H. Purwaningsih, Nurhadi Ibrahim and Hamdani Zain
Students : ‐
Sponsors : RUUI 2007
Email contact : erniepoerwa@yahoo.com, nurhadifkui87@yahoo.com

The studies of the neuro‐protection and neuro‐therapy of Acalypha indica Linn. extract have already done. This extract has also proven as neuro‐protection and neurotherapy, both ex vivo on m. gastrocnemius of frog and in vivo on frog, beside antiurosemic and anti‐diabetic’s effect. The experimental studies were done on 4 groups of frog, 2 groups for ex vivo and 2 groups for in vivo studies. The ex vivo groups divided into 7 subgroups of application, 4 samples each. There were 5 subgroups of doses: 5; 10; 15; 20; 25 mg and 2 subgroups as control. For in vivo study, there were 6 subgroups of application (2 subgroups as control, 4 subgroups of doses 6; 9; 12; 15 mg/frog). Pancuronium Bromide 0.2 %, 4 mg, is used for muscle relaxant. The parameters which have measured in these studies were the electrical activities such as amount and duration (second) of re‐polarization; depolarization, resting potential, and the high of spike after stimulation.

The dose of extract Acalypha indica Linn. of 15 mg and 20 mg/mL have shown better activities than the lower dose or 25 mg of extract, both as neuro‐protection ( Ringer ‐ Extract ‐ Pancuronium Bromide ) and neuro‐therapy (Ringer – Pancuronium Bromide – Extract). In in vivo studies, the administration of extract 9 mg – 15 mg, orally, were proven as neuro‐protection before injecting with Pankuronium Bromide 0.2 % (1:40) and neurotherapy, after injecting with Pankuronium Bromide 0.2 % (1:40). The effects of their recovery time (minutes), more effective compare to their negative control, but have not significant different between these dose and the positive control of Piracetam 50 mg/Kg.BW.

Keywords: neuro‐protection, neuro‐therapy, Acalypha indica Linn. ex vivo, in vivo.

[Kembali]